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Production and Purification of Antibodies from Hybridoma Lines
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/00000138-80f5-1727-fbab-3b8480000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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Produce and purify antibodies from culture supernatants or ascites. Hybridoma cells can be grown either in roller bottles or in hollow fiber cartridges. The culture supernatant (1 liter or more) is precipitated by ammonium sulphate and IgG is purified by protein G chromatography through FPLC.
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Service Fee URL
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http://www.med.upenn.edu/genetics/cellctr/services/cell_charges.shtml
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Additional Name
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Hybridoma production and screening
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Service Provided by
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Cell Center Services Facility (Penn)
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Website(s)
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http://www.med.upenn.edu/genetics/cellctr/services/cell_culture_services.shtml
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Related Technique
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Antibody production
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Related Technique
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Antibody purification
