Production and Purification of Antibodies from Hybridoma Lines

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   Produce and purify antibodies from culture supernatants or ascites. Hybridoma cells can be grown either in roller bottles or in hollow fiber cartridges. The culture supernatant (1 liter or more) is precipitated by ammonium sulphate and IgG is purified by protein G chromatography through FPLC.

3. Service Fee URL

   http://www.med.upenn.edu/genetics/cellctr/services/cell_charges.shtml

4. Additional Name

   Hybridoma production and screening

5. Service Provided by

   Cell Center Services Facility (Penn)

6. Website(s)

   http://www.med.upenn.edu/genetics/cellctr/services/cell_culture_services.shtml

7. Related Technique

   Antibody production

8. Related Technique

   Antibody purification