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Mycoplasma testing using MycoAlert

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-817c-59a4-9cd7-d7e280000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "This test is extremely rapid, highly sensitive and is generic for all common mycoplasma strains. Mycoplasma have unique metabolic processes that derive energy from their host cells. The method specifically detects enzymes involved in mycoplasma metabolism that are absent in host cells. By using substrates for the mycoplasma specific enzymes, ADP is converted to ATP. The resultant ATP is conveniently detected using the bioluminescent reaction catalyzed by the Firefly Luciferase enzyme. Any increase in light output from this reaction, as measured by a luminometer, will indicate the presence of mycoplasma. We run a mycoplasma test every Friday morning. Please note that samples should arrive at our facility by Thursday afternoon before 4:30 pm to be included in Friday’s testing. You will receive results that Friday afternoon."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/cellctr/services/cell_charges.shtml
  4. Contact
    Baxter, Sabine
  5. Service Provided by
    Cell Center Services Facility (Penn)
  6. Website(s)
    http://www.med.upenn.edu/genetics/cellctr/services/cell_culture_services.shtml
  7. Related Technique
    Mycoplasma testing
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-07-13T12:58:22.781-05:00
  4. creator
    apizarro (Angel Pizarro)
  5. modified
    2016-04-22T13:18:17.224-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016