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EBV Transformation

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-8180-bdce-9cd7-d7e280000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Epstein Barr Virus (EBV) transformation is a reliable method to immortalize mammalian cells. It is most often used to obtain cell lines from human lymphocytes that serve as a permanent source for DNA isolation and protein isolation. The technique has found widespread use in clinical trials as the principal method of generating a permanent source of patient DNA for genotyping. Patient genotyping is becoming more popular as the genetic causes of diseases are being unraveled at increasing speed. EBV is the causative agent of human infectious mononucleosis , a childhood disease. More than 90% of adults are a carrier of EBV. Adults usually show no EBV symptoms. EBV is a Herpesvirus and its genome consists of a 172kb linear double stranded DNA which has been completely sequenced. EBV molecular biology and pathogenesis are extensively studied and the roles of many crucial EBV and host cell genes in pathogenesis are known. EBV infects only certain mammalian epithelial cells and B lymphocytes. In vitro EBV immortalizes B-cells by activating a number of cell cycle regulating genes as well as B-cell specific genes including immunoglobulin genes. Usually B-cell infections are latent and only strong stimulation can cause the lytic cycle. EBV DNA is replicated as an episomal ring and 10-20 copies per cell are typical. IMPORTANT: When working with EBV (or any other infectious agent) negative control blood samples may NOT be obtained from laboratory personnel in order to minimize the risk of infections with one's own EBV-transformed blood cells against which the body would not be able to mount an immune response."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/cellctr/services/cell_charges.shtml
  4. Service Provided by
    Cell Center Services Facility (Penn)
  5. Website(s)
    http://www.med.upenn.edu/genetics/cellctr/services/cell_culture_services.shtml
  6. Related Technique
    Cell immortalization
  7. Related Technique
    EBV transformation
 
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Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016