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Specimen preparation for electron microscopy

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-9bb1-739f-9cd7-d7e280000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    The facility’s Abra HPM010 high pressure freezer, two Leica AFSII freeze substitution machines, Leica CPM100 plunge/slam freezer, and cryogenic sectioning microtomes are available for cryogenic preparation of biological material and give superior preservation of biological specimens. In addition, our staff includes three dedicated personal with over fifty combined years of experience and equipment for all types of electron microscopy sample preparation at a nominal cost to U Penn researchers. Preparation services for of all types of biological specimens for electron microscopy analysis include: • plunge freezing • high pressure freezing • freeze substitution fixation • microwave tissue processing • thin sectioning • thick sectioning • cryosectioning • cryo-ultrasectioning • pre-embedding immunogold labeling • post-embedding immunogold labeling • SEM sample preparation/critical point drying
  3. Service Fee URL
    https://somapps.med.upenn.edu/pbr/portal/morph/fees.php
  4. Additional Name
    Specimen processing
  5. Related Resource
    Abra HPM010
  6. Related Resource
    Leica AFSII
  7. Related Resource
    Leica CPM100 plunge/slam freezer
  8. Related Resource
    Reichert Ultracut-S
  9. Service Provided by
    Electron Microscopy Resource Laboratory (Penn)
  10. Website(s)
    https://pathbio.med.upenn.edu/pbr/portal/morph/tech.php
  11. Related Technique
    Cryosectioning
  12. Related Technique
    Transmission electron microscopy
  13. Related Technique
    Tissue embedding
  14. Related Technique
    Tissue sectioning
  15. Related Technique
    Immunogold labeling
  16. Related Technique
    Fixation
  17. Related Technique
    Staining
  18. Related Technique
    Polymerization
  19. Related Technique
    Cell fixation
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-07-18T15:48:57.765-05:00
  4. creator
    fcoldren
  5. modified
    2016-04-25T15:39:04.876-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016