eagle-i The University of PennsylvaniaThe University of Pennsylvania
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

Large sequencing project support

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-de14-01bc-eb5b-b63c80000000

Resource Type

  1. Support service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The facility will sequence through DNA clones by primer walking or, if needed, deletion subcloning. Please fill out a Request for Sequencing Project Management form when submitting the sample, indicating if you wish to select primer sequences yourself or delegate the responsibility to facility staff. Charges for primer walks are calculated according to the number of lanes sequenced and primers constructed. A clean-up treatment of sufficient template for the whole project (nominally 4-5 µg per kb for each direction) is recommended to produce quality sequence and maximize step size. After good quality template is obtained, each step (~500 nucl.) requires around one week, including primer selection and synthesis, sequencing, sequence evaluation, and assembly. Charges for deletion subcloning should be discussed with facility staff. Assembly of the overall sequence, ORF analysis, and homology searches can be performed, at no charge, if requested by the user."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/dnaseq/pricing.shtml
  4. Service Provided by
    DNA Sequencing Facility (Penn)
  5. Website(s)
    http://www.med.upenn.edu/genetics/dnaseq/sanger_proj.shtml
  6. Related Technique
    DNA sequencing assay
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-07-31T12:32:34.837-05:00
  4. creator
    fcoldren
  5. modified
    2013-08-23T14:19:17.545-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016