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Genotyping and fragment analysis service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-de40-5c97-eb5b-b63c80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Genotyping/Fragment sizing is currently done on ABI (Applied Biosystems) 3130XL capillary sequencer, for microsatellite-based genotyping used in linkage analysis, identifying VNTR alleles in a genetic screen, mapping genomic clones, or any other purposes. For this PCR products need to be generated with one primer labeled on the 5′ side preferably with an ABI synthesized dye FAM, HEX (or VIC), NED etc (see below). Submit about 3-4 μl PCR products (single or pooled) in 0.2 μl strip tubes or in a reaction plate. Samples can also be submitted in a ready-to-load format (PCR products + ROX size standard + formamide already added) on an ABI 96-well reaction plate."
  3. Related Resource
    ABI 3130XL DNA analyzer
  4. Related Resource
    ABI GeneMapper
  5. Service Provided by
    DNA Sequencing Facility (Penn)
  6. Website(s)
    http://www.med.upenn.edu/genetics/dnaseq/genotyping.shtml
  7. Related Technique
    Single-nucleotide polymorphism analysis
  8. Related Technique
    Microsatellite analysis
  9. Related Technique
    Genotyping assay
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-07-31T13:22:52.390-05:00
  4. creator
    fcoldren
  5. modified
    2016-04-22T20:24:47.646-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016