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Mouse genotyping service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-de54-93a9-eb5b-b63c80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Rapid genotyping of transgenic mice is highly important for successful breeding as well as for reducing the cost of strain maintenance. Particularly for large and multiple litters one needs to have the genotyping performed quickly in a cost-effective manner. We offer a fast and convenient service for large scale mouse genotyping using Sigma REDExtract-N-Amp Tissue PCR kit starting from tail biopsies or ear punches. The user provides the mouse tails, control DNA with the transgene in it, and the PCR primers for the transgene together with any special PCR protocol. Two PCRs are done for each sample, one for the transgene and the other a control mGAPDH fragment. The amplicons are run on agarose gel and the labeled gel electrophoresis picture is provided within 48 hrs."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/dnaseq/pricing.shtml
  4. Topic
    Mus musculus
  5. Service Provided by
    DNA Sequencing Facility (Penn)
  6. Website(s)
    http://www.med.upenn.edu/genetics/dnaseq/mousegeno.shtml
  7. Related Technique
    Transgenic mouse model generation
  8. Related Technique
    Genotyping assay
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-07-31T13:40:04.551-05:00
  4. creator
    fcoldren
  5. modified
    2016-04-22T20:28:44.207-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016