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iPSC derivation from fibroblasts using lentiviral vector or Sendai virus

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-e365-6c8b-eb5b-b63c80000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The Core accepts either live or frozen cell stocks. Submitted cells MUST be mycoplasma free before they are accepted. Cells should also be at low passages (preferably <10) and are proliferating as non-dividing cells are refractory to reprogramming. This service takes 3-6 months to complete and includes the following: • Expansion, mycoplasma testing, cryopreservation of fibroblast culture • Transduction of fibroblasts with lentiviral vector or Sendai viral vectors • iPSC colony picking • Expansion of 4 iPSC clones • Mycoplasma testing • FACS analysis of 2 pluripotency markers (TRA-1-60 and SSEA4) • Cryopreservation of iPSC lines (4-6 vials/clone) • Will deliver 4 clones (frozen or live culture) at passage >5"
  3. Service Fee URL
    http://www.med.upenn.edu/ipsccore/services.shtml
  4. Additional Name
    Derivation of iPSCs
  5. Service Provided by
    Induced Pluripotent Stem Cell Core Facility (Penn)
  6. Website(s)
    http://www.med.upenn.edu/ipsccore/services.shtml
  7. Related Protocol(s)
    Lentivirus generation protocol
  8. Related Protocol(s)
    Mouse embryonic and induced pluripotent stem cell culture
  9. Related Technique
    Viral infection
  10. Related Technique
    Mycoplasma testing
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-08-01T13:22:46.158-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-19T17:22:32.856-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016