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iPSC derivation from fibroblasts using lentiviral vector or Sendai virus

eagle-i ID


Resource Type

  1. Material production service


  1. Fee for service
  2. Resource Description
    "The Core accepts either live or frozen cell stocks. Submitted cells MUST be mycoplasma free before they are accepted. Cells should also be at low passages (preferably <10) and are proliferating as non-dividing cells are refractory to reprogramming. This service takes 3-6 months to complete and includes the following: • Expansion, mycoplasma testing, cryopreservation of fibroblast culture • Transduction of fibroblasts with lentiviral vector or Sendai viral vectors • iPSC colony picking • Expansion of 4 iPSC clones • Mycoplasma testing • FACS analysis of 2 pluripotency markers (TRA-1-60 and SSEA4) • Cryopreservation of iPSC lines (4-6 vials/clone) • Will deliver 4 clones (frozen or live culture) at passage >5"
  3. Service Fee URL
  4. Additional Name
    Derivation of iPSCs
  5. Service Provided by
    Induced Pluripotent Stem Cell Core Facility (Penn)
  6. Website(s)
  7. Related Protocol(s)
    Lentivirus generation protocol
  8. Related Protocol(s)
    Mouse embryonic and induced pluripotent stem cell culture
  9. Related Technique
    Viral infection
  10. Related Technique
    Mycoplasma testing
Provenance Metadata About This Resource Record
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  2. contributor
  3. created
  4. creator
  5. modified

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016