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Characterization and validation services

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000138-e370-8818-eb5b-b63c80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Newly derived iPS cell lines can be transferred to the investigator without further characterization and validation or they can be further expanded and characterized at the Core. The Core will ask the PI to make a decision for characterization and validation services before freezing down all newly derived iPSC cultures. <strong>New iPSC line characterization</strong> This service takes about 1 month to complete and the following assays are performed: • Immunocytochemistry for 4 pluripotency markers • qRT-PCR analysis for Oct4, Sox2, Nanog, Rex1, and Dmnt3b • Embryoid body formation and qRT-PCR analysis for 2 differentiation markers of each of the three cell lineages <strong>Karyotyping</strong> iPS cell culture will be expanded and live culture will be sent to Cell Line Genetics for G-band karyotyping. <strong>DNA finger printing</strong> Feeder-free iPS cell culture will be expanded and cells will be harvested. Genomic DNA will be isolated from iPS cells and the corresponding parental cell line. DNA will be sent to Cell Line Genetics for Short Tandem Repeat (STR) analysis for originating vs. resulting cell verification."
  3. Service Fee URL
    http://www.med.upenn.edu/ipsccore/services.shtml
  4. Additional Name
    Characterization of iPS cells
  5. Service Provided by
    Induced Pluripotent Stem Cell Core Facility (Penn)
  6. Website(s)
    http://www.med.upenn.edu/ipsccore/services.shtml
  7. Related Technique
    Microsatellite analysis
  8. Related Technique
    Real-time PCR
  9. Related Technique
    Immunocytochemistry
  10. Related Technique
    Karyotyping assay
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-08-01T13:31:06.235-05:00
  4. creator
    fcoldren
  5. modified
    2015-02-19T12:48:02.478-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016