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Characterization and validation services

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    "Newly derived iPS cell lines can be transferred to the investigator without further characterization and validation or they can be further expanded and characterized at the Core. The Core will ask the PI to make a decision for characterization and validation services before freezing down all newly derived iPSC cultures. <strong>New iPSC line characterization</strong> This service takes about 1 month to complete and the following assays are performed: • Immunocytochemistry for 4 pluripotency markers • qRT-PCR analysis for Oct4, Sox2, Nanog, Rex1, and Dmnt3b • Embryoid body formation and qRT-PCR analysis for 2 differentiation markers of each of the three cell lineages <strong>Karyotyping</strong> iPS cell culture will be expanded and live culture will be sent to Cell Line Genetics for G-band karyotyping. <strong>DNA finger printing</strong> Feeder-free iPS cell culture will be expanded and cells will be harvested. Genomic DNA will be isolated from iPS cells and the corresponding parental cell line. DNA will be sent to Cell Line Genetics for Short Tandem Repeat (STR) analysis for originating vs. resulting cell verification."
  3. Service Fee URL
  4. Additional Name
    Characterization of iPS cells
  5. Service Provided by
    Induced Pluripotent Stem Cell Core Facility (Penn)
  6. Website(s)
  7. Related Technique
    Microsatellite analysis
  8. Related Technique
    Real-time PCR
  9. Related Technique
  10. Related Technique
    Karyotyping assay
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016