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Qubit fluorometer

eagle-i ID


Resource Type

  1. Fluorometer


  1. Resource Description
    "The NGSC uses the Life Technologies QuBit to assess the concentration of libraries. The Qubit can measure protein, single- and double-stranded DNA, and RNA. The single-stranded DNA mode is especially helpful to quantify RNA-seq libraries with the 'high junk'. This high junk is good library and is an indication of over PCRing or not enough primers. Since the ends of the library have sequence homology, they will substitute for the primers and thus cause daisy chaining or a big clump of ssDNA. This is the high junk. Therefore, denaturing the libraries and using the ssDNA Qubit kit allows us to resolve the actual concentration of these complicated libraries."
  2. Manufacturer
  3. Website(s)
  4. Website(s)
  5. Related Technique
    DNA quantitation assay
  6. Related Technique
    RNA quantitation assay
  7. Related Technique
    RNA quality analysis
  8. Related Technique
    Protein quantitation assay
  9. Related Technique
    DNA quality analysis
  10. Related Technique
    Quality control
  11. Location
    Next-Generation Sequencing Core (Penn)
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016