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Qubit fluorometer
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/00000139-49c8-8de7-eb5b-b63c80000000
Resource Type
Properties
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Resource Description
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"The NGSC uses the Life Technologies QuBit to assess the concentration of libraries. The Qubit can measure protein, single- and double-stranded DNA, and RNA. The single-stranded DNA mode is especially helpful to quantify RNA-seq libraries with the 'high junk'.
This high junk is good library and is an indication of over PCRing or not enough primers. Since the ends of the library have sequence homology, they will substitute for the primers and thus cause daisy chaining or a big clump of ssDNA. This is the high junk. Therefore, denaturing the libraries and using the ssDNA Qubit kit allows us to resolve the actual concentration of these complicated libraries."
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Manufacturer
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Invitrogen
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Website(s)
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http://fgc-ngsc-cores.blogspot.com/search?q=qubit
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Website(s)
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http://www.invitrogen.com/site/us/en/home/brands/Product-Brand/Qubit.html
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Related Technique
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DNA quantitation assay
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Related Technique
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RNA quantitation assay
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Related Technique
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RNA quality analysis
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Related Technique
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Protein quantitation assay
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Related Technique
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DNA quality analysis
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Related Technique
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Quality control
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Location
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Next-Generation Sequencing Core (Penn)
