Qubit fluorometer

eagle-i ID

Resource Type

Properties

1. Resource Description

   "The NGSC uses the Life Technologies QuBit to assess the concentration of libraries. The Qubit can measure protein, single- and double-stranded DNA, and RNA. The single-stranded DNA mode is especially helpful to quantify RNA-seq libraries with the 'high junk'. This high junk is good library and is an indication of over PCRing or not enough primers. Since the ends of the library have sequence homology, they will substitute for the primers and thus cause daisy chaining or a big clump of ssDNA. This is the high junk. Therefore, denaturing the libraries and using the ssDNA Qubit kit allows us to resolve the actual concentration of these complicated libraries."

2. Manufacturer

   Invitrogen

3. Website(s)

   http://fgc-ngsc-cores.blogspot.com/search?q=qubit

4. Website(s)

   http://www.invitrogen.com/site/us/en/home/brands/Product-Brand/Qubit.html

5. Related Technique

   DNA quantitation assay

6. Related Technique

   RNA quantitation assay

7. Related Technique

   RNA quality analysis

8. Related Technique

   Protein quantitation assay

9. Related Technique

   DNA quality analysis

10. Related Technique

    Quality control

11. Location

    Next-Generation Sequencing Core (Penn)