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Bio-Rad Radiance 2100

eagle-i ID


Resource Type

  1. Confocal microscope


  1. Related Software
    Bio-Rad Lasersharp 2000
  2. Resource Description
    "Penn Dental Medicine houses a Radiance 2100 laser confocal microscope with argon, green He/Ne, red diode, and blue diode lasers available for use by researchers throughout the School as well as others inside and outside the University. Three photomultiplier tubes (PMTs) are available for capture of up to three distinct fluorescent emissions simultaneously. With this laser configuration, the microscope is equipped to capture most fluors with excitations ranging from 340 to 650 and emission from 400 to over 700, allowing co-localization of three fluorescent markers in a single image, with the capability of capturing up to six distinct fluorescent signatures per image. The microscope attached to the confocal is an inverted Nikon microscope equipped with 40X air, 60X oil, and 100X oil objectives and filters to view fluors with emission peaks ranging from 450 to over 700. The scope is also outfitted with Differential Interference Contrast (DIC) optics for transmission images of the tissue or cells as well as 10X, 20X, and 40X phase objectives with an associated long working distance lens and phase rings to capture transmission images by phase microscopy. This combination of objectives, light imaging, and fluorescent filters can serve a variety of functions."
  3. Manufacturer
    Bio-Rad Laboratories, Inc.
  4. Website(s)
  5. Related Technique
    Confocal microscopy
  6. Related Technique
    Time lapse confocal microscopy
  7. Related Technique
    Fluorescent microscopy
  8. Related Technique
    Phase contrast microscopy
  9. Related Technique
    Differential interference contrast microscopy
  10. Location
    Laser Confocal Microscope Core (Penn)
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016