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Affymetrix GeneChip Instrument System
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/00000139-6985-c3e5-4778-6c3080000000
Resource Type
Properties
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Resource Description
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"Gene expression profiling or microarray analysis has enabled the measurement of thousands of genes in a single RNA sample. GeneChip microarrays are manufacture by photochemical synthesis. Because of this manufacturing technology, more than a million different probes can be synthesized on an array roughly the size of a thumbnail. These numbers allow the inclusion of multiple probes to interrogate the same target sequence, providing statistical rigor to data interpretation. Over the years the GeneChip platform has proven to be a reliable and robust system, enabling many new discoveries and breakthroughs to be made by the scientific community.
After hybridization of the biotinylated sample, the chip is stained with streptavidin-phycoerythrin and read with a confocal scanner. Control and experimental samples are hybridized to separate chips. Comparison of the two chips is performed to determine the differential gene expression levels of the two compared samples."
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Manufacturer
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Affymetrix, Inc.
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Website(s)
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https://somapps.med.upenn.edu/pbr/portal/mpf/technology.php#genechipsequenom
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Related Technique
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SNP genotyping assay
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Related Technique
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Affymetrix array
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Related Technique
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miRNA expression assay
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Location
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Molecular Profiling Facility (Penn)
