Affymetrix GeneChip Instrument System

eagle-i ID

Resource Type

Properties

1. Resource Description

   "Gene expression profiling or microarray analysis has enabled the measurement of thousands of genes in a single RNA sample. GeneChip microarrays are manufacture by photochemical synthesis. Because of this manufacturing technology, more than a million different probes can be synthesized on an array roughly the size of a thumbnail. These numbers allow the inclusion of multiple probes to interrogate the same target sequence, providing statistical rigor to data interpretation. Over the years the GeneChip platform has proven to be a reliable and robust system, enabling many new discoveries and breakthroughs to be made by the scientific community. After hybridization of the biotinylated sample, the chip is stained with streptavidin-phycoerythrin and read with a confocal scanner. Control and experimental samples are hybridized to separate chips. Comparison of the two chips is performed to determine the differential gene expression levels of the two compared samples."

2. Manufacturer

   Affymetrix, Inc.

3. Website(s)

   https://somapps.med.upenn.edu/pbr/portal/mpf/technology.php#genechipsequenom

4. Related Technique

   SNP genotyping assay

5. Related Technique

   Affymetrix array

6. Related Technique

   miRNA expression assay

7. Location

   Molecular Profiling Facility (Penn)