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Affymetrix GeneChip Instrument System

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000139-6985-c3e5-4778-6c3080000000

Resource Type

  1. Microarray platform

Properties

  1. Resource Description
    "Gene expression profiling or microarray analysis has enabled the measurement of thousands of genes in a single RNA sample. GeneChip microarrays are manufacture by photochemical synthesis. Because of this manufacturing technology, more than a million different probes can be synthesized on an array roughly the size of a thumbnail. These numbers allow the inclusion of multiple probes to interrogate the same target sequence, providing statistical rigor to data interpretation. Over the years the GeneChip platform has proven to be a reliable and robust system, enabling many new discoveries and breakthroughs to be made by the scientific community. After hybridization of the biotinylated sample, the chip is stained with streptavidin-phycoerythrin and read with a confocal scanner. Control and experimental samples are hybridized to separate chips. Comparison of the two chips is performed to determine the differential gene expression levels of the two compared samples."
  2. Manufacturer
    Affymetrix, Inc.
  3. Website(s)
    https://somapps.med.upenn.edu/pbr/portal/mpf/technology.php#genechipsequenom
  4. Related Technique
    SNP genotyping assay
  5. Related Technique
    Affymetrix array
  6. Related Technique
    miRNA expression assay
  7. Location
    Molecular Profiling Facility (Penn)
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-08-27T14:20:22.904-05:00
  4. creator
    fcoldren
  5. modified
    2014-10-28T14:52:00.202-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016