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Total internal reflection fluorescence atomic force microscope

eagle-i ID


Resource Type

  1. Total Internal Reflection Fluorescence Atomic force microscope


  1. Resource Description
    AFM with 16 μm vertical range and 90 μm lateral range. Also excels at 1-D pulling or pushing force measurements. Mounted on inverted optical microscope with excitation from three lasers: red (Coherent CUBE 641 nm, 100 mW) green (Crystalaser CL-532 nm, 100 mW) blue (Coherent Sapphire 488 nm, 75 mW) Environmental control includes heated environmental enclosure and Bio-heater fluid cell: AFM and optical microscope can be heated up to 37ºC Eppendorf micro-manipulator and pipetter for in situ dosing of cells and tissues. 10x, 20x, 40x and 100x lenses with high-speed camera allow viewing of AFM tip in contact with sample. Sample application: co-localization of actin filaments by optical microscopy and AFM
  2. Additional Name
    Asylum MFP-3D TIRF AFM
  3. Contact
    Brukman, Matt., PhD
  4. Manufacturer
    Asylum Research
  5. Related Publication or Documentation
    Hyaluronan and dextran modified tubes resist cellular activation with blood contact
  6. Website(s)
  7. Website(s)
  8. Related Technique
    Light microscopy
  9. Related Technique
    Atomic force microscopy
  10. Related Technique
    Fluorescent microscopy
  11. Location
    NBIC Probe Facility (Penn)
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016