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Total internal reflection fluorescence atomic force microscope

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/00000139-b6ca-59da-9dbb-bb2180000000

Resource Type

  1. Total Internal Reflection Fluorescence Atomic force microscope

Properties

  1. Resource Description
    AFM with 16 μm vertical range and 90 μm lateral range. Also excels at 1-D pulling or pushing force measurements. Mounted on inverted optical microscope with excitation from three lasers: red (Coherent CUBE 641 nm, 100 mW) green (Crystalaser CL-532 nm, 100 mW) blue (Coherent Sapphire 488 nm, 75 mW) Environmental control includes heated environmental enclosure and Bio-heater fluid cell: AFM and optical microscope can be heated up to 37ºC Eppendorf micro-manipulator and pipetter for in situ dosing of cells and tissues. 10x, 20x, 40x and 100x lenses with high-speed camera allow viewing of AFM tip in contact with sample. Sample application: co-localization of actin filaments by optical microscopy and AFM
  2. Additional Name
    Asylum MFP-3D TIRF AFM
  3. Contact
    Brukman, Matt, Ph.D.
  4. Manufacturer
    Asylum Research
  5. Related Publication or Documentation
    Hyaluronan and dextran modified tubes resist cellular activation with blood contact
  6. Website(s)
    http://www.nanotech.upenn.edu/facilities_images/TowneTIRF-AFM.pdf
  7. Website(s)
    http://www.nanotech.upenn.edu/instrumentation.html
  8. Related Technique
    Light microscopy
  9. Related Technique
    Atomic force microscopy
  10. Related Technique
    Fluorescent microscopy
  11. Location
    NBIC Probe Facility (Penn)
 
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016