eagle-i The University of PennsylvaniaThe University of Pennsylvania
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

Embryonic stem cell injection

eagle-i ID


Resource Type

  1. Material processing service


  1. Fee for service
  2. Resource Description
    "The production of chimeric mice by introducing Embryonic Stem (ES) cells into early embryos is an alternative approach to generating transgenic mice. The technique utilizes the tissue culture system to modify and select ES cells that had received an exogenous DNA of interest. Once derived and characterized, ES cell clones may be transferred into 4-day-old mouse embryos where they can differentiate into any adult tissue type. <strong>What the Facility will Provide</strong> Through this service, the facility will generate chimeric mice by injecting Embryonic Stem (ES) cells containing site specific genomic alterations (knockouts) into normal blastocysts. ES cells will be injected into a minimum of 50 blastocysts of C57BL/6J or Balb/c. When the blastocysts have reexpanded, they will be transferred into the uterine horn of pseudopregnant female and allowed to go to term. Upon weaning 3 weeks after birth, chimeric mice are separated and then released to the care of the investigator."
  3. Service Fee URL
  4. Additional Name
    Blastocyst microinjections
  5. Additional Name
    ES cell injection
  6. Contact
    Richa, Jean., PhD
  7. Topic
    Mus musculus
  8. Service Provided by
    Transgenic and Chimeric Mouse Facility (Penn)
  9. Website(s)
  10. Related Technique
    Blastocyst injection
  11. Related Technique
    Transgenic mouse model generation
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
  3. created
  4. creator
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016