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Embryonic stem cell injection

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013a-5163-286d-01af-beb880000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The production of chimeric mice by introducing Embryonic Stem (ES) cells into early embryos is an alternative approach to generating transgenic mice. The technique utilizes the tissue culture system to modify and select ES cells that had received an exogenous DNA of interest. Once derived and characterized, ES cell clones may be transferred into 4-day-old mouse embryos where they can differentiate into any adult tissue type. <strong>What the Facility will Provide</strong> Through this service, the facility will generate chimeric mice by injecting Embryonic Stem (ES) cells containing site specific genomic alterations (knockouts) into normal blastocysts. ES cells will be injected into a minimum of 50 blastocysts of C57BL/6J or Balb/c. When the blastocysts have reexpanded, they will be transferred into the uterine horn of pseudopregnant female and allowed to go to term. Upon weaning 3 weeks after birth, chimeric mice are separated and then released to the care of the investigator."
  3. Service Fee URL
    http://www.med.upenn.edu/genetics/tcmf/charges.shtml
  4. Additional Name
    Blastocyst microinjections
  5. Additional Name
    ES cell injection
  6. Contact
    Richa, Jean, Ph.D.
  7. Topic
    Mus musculus
  8. Service Provided by
    Transgenic and Chimeric Mouse Facility (Penn)
  9. Website(s)
    http://www.med.upenn.edu/genetics/tcmf/services_ES_cell.shtml
  10. Related Technique
    Blastocyst injection
  11. Related Technique
    Transgenic mouse model generation
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-10-11T14:58:23.228-05:00
  4. creator
    fcoldren
  5. modified
    2016-04-21T13:34:54.178-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016