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Mouse line re-derivation

eagle-i ID


Resource Type

  1. Material production service


  1. Fee for service
  2. Resource Description
    "This process involves the surgical transfer of embryos into pathogen-free recipient females, where they can develop to term. Rederivation is the most effective method to rid a colony of pathogenic infections. It is also recommended when genetically altered animals are acquired from outside institutions that do not fall into the category of approved vendors. With the proper procedure, mouse lines can be saved or transferred safely across distant locations. Due to the diverse environmental conditions among the animal facilities located across campus, the structure of this service will be tailored to fit each and every facility receiving the rederived line. As a consequence, the fees may vary accordingly. Basically, a total of 50 embryos generated from the designated mouse line will be transferred into recipient pathogen-free females that would carry them to term in a housing location assigned by ULAR. The mice born from such transfers should be of sufficient number to rejuvenate or establish the line." <b>Alternative service for re-derivation: IVF</b> "The epididymis of the donor male will be harvested by the user (as in 1., above), delivered to the TCMF, and the released sperm will be used for IVF of wild-type oocytes. The oocytes will have to be harvested in the TCMF from females acquired from approved vendors. Fertilized oocytes will be cultured overnight and any resulting 2-cell stage embryos will be transferred into surrogate females. The transplanted pseudo-pregnant recipients will then be then transferred to the Levy building for gestation and quarantine monitoring. ULAR diagnostics must be contacted and space within the Levy quarantine cleared before any date for IVF can be set. The user should consult with ULAR regarding duration and rules of quarantine in Levy." <b>Alternative service for re-derivation: Embryo transfer</b> "This requires hands-on training of lab personnel to perform intraperitoneal (ip) hormone injection and to dissect uterine horns. All media and hormones will be supplied by the facility as will the actual training. The user’s lab will be responsible for two ip hormone injections, 48 hrs apart between the hours of 12 PM and 2 PM, into a set of 3-week old females. Once the second hormone injection is complete the female mice should be housed with single caged males and left alone for 3.5 days. On the morning of harvest, the females will be sacrificed by the user, uterine horns removed and a lab member who has not been near the actual animals must deliver the organs to the TCMF (behind the microbiological barrier) in doubled Petri dishes. ULAR diagnostics must be contacted and space within the Levy quarantine cleared before any date for embryo harvest can be set. The user should consult with ULAR regarding duration and rules of quarantine in Levy."
  3. Service Fee URL
  4. Contact
    Richa, Jean., PhD
  5. Topic
    Mus musculus
  6. Service Provided by
    Transgenic and Chimeric Mouse Facility (Penn)
  7. Website(s)
  8. Website(s)
  9. Related Technique
    Embryo transplantation
  10. Related Technique
    Transgenic mouse model generation
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016