Nanodrop 1000 spectrophotometer

eagle-i ID

Resource Type

Properties

1. Resource Description

   "The Thermo Scientific NanoDropTM 1000 Spectrophotometer measures 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer). <b>Applications</b> • Nucleic acid concentration and purity of nucleic acid samples up to 3700 ng/ul (dsDNA) without dilution • Fluorescent dye labeling density of nucleic acid microarray samples • Purified protein analysis (A280) up to 100 mg/ml (BSA) • Expanded spectrum measurement and quantitation of fluorescent dye labeled proteins, conjugates, and metalloproteins • Bradford Assay analysis of protein • BCA Assay analysis of protein • Lowry Assay analysis of protein • Pierce Protein 660 nm Protein Assay • Cell density measurements • General UV-Vis spectrophotometry" <i>excerpt from <b>NanoDrop 1000 Spectrophotometer v3.7 User's Manual</b></i>

2. Manufacturer

   Thermo Fisher Scientific

3. Website(s)

   http://nanodrop.com/Library/nd-1000-v3.7-users-manual-8.5x11.pdf

4. Related Technique

   RNA quality analysis

5. Related Technique

   DNA quality analysis

6. Related Technique

   Spectrophotometry

7. Location

   The Genotyping and DNA/RNA Analysis Core (Monell)