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Nr4aDN mice

eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    Nr4a family gene expression increases within the hippocampus after training in a hippocampus-dependent task. The dominant-negative form of NR4A1 (NR4ADN) contains the DNA-binding and dimerization domains but lacks the transactivation domain. It silences the three Nr4a family members. We generated a transgenic mouse line expressing the NR4ADN construct under control of the tetracycline operator (tetO), which we combined with the CaMKII–tetracycline transactivator (CaMKII-tTA) transgene to achieve expression selectively within postnatal excitatory forebrain neurons. The dominant-negative protein interacts with NR4A proteins in vivo. Transgene expression was restricted in the forebrain to the striatum, sparse cortical areas, and subregions of the hippocampal formation (CA1 and the dentate gyrus). Transgene expression was not observed in the amygdala or in area CA3 of the hippocampus. Nr4a polymorphisms have been identified in patients with schizophrenia, and Nr4a gene expression is reduced in patients with schizophrenia. Thus, impaired Nr4a function may contribute to the cognitive impairments that accompany this psychiatric disorder.
  2. Additional Name
    Nr4a dominant-negative mice
  3. Related Disease
  4. Related Publication or Documentation
    NR4A nuclear receptors support memory enhancement by histone deacetylase inhibitors
  5. Parental Strain Name
  6. Biological process studied
  7. Biological process studied
    Long-term memory
  8. Genetic Alteration(s)
    tetO-YFP-HA-NR4ADN insertion
  9. Phenotype Findings
    Impaired long-term contextual memory consolidation
  10. Location
    Ted Abel Laboratory
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016