COX-2 Y385F

eagle-i ID

Resource Type

Properties

1. Resource Description

   We introduced a point mutation (1121a→t) into the Ptgs2 gene (which encodes PGHS2) of mice by gene targeting, resulting in a Y385F substitution, to create a genetic mimic of selective COX-2 inhibition (cycloxygenase activity is inhibited, but peroxidase activity is intact). We numbered the tyrosine residue of PGHS2 in accordance with the numbering of sheep PGHS1, the recognized standard for numbering amino acid residues of PHGS isoforms. Accordingly, the actual position of the affected tyrosine residue in mouse PGHS2 is Tyr371 based on numbering the amino-terminal methionine of the signal peptide as residue 1.

2. Contact

   Teegarden, Sarah, Ph.D.

3. Related Disease

   D009358

4. Related Disease

   D020763

5. Related Disease

   cardiovascular system disease

6. Related Disease

   female reproductive system disease

7. Related Disease

   placenta disease

8. Related Publication or Documentation

   Cyclooxygenases, microsomal prostaglandin E synthase-1, and cardiovascular function

9. Related Publication or Documentation

   Genetic model of selective COX2 inhibition reveals novel heterodimer signaling

10. Genetic Alteration(s)

    COX-2 Y385F

11. Phenotype Findings

    Closure of ductus arteriosus

12. Phenotype Findings

    Abnormal renal development

13. Phenotype Findings

    Reproductive phenotype

14. Phenotype Findings

    Cardiovascular phenotype

15. Location

    FitzGerald Lab