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Quality control standard operating procedures for AAV vectors, Ad vectors, and Lentiviral vectors

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013b-66c4-1916-83a0-df0880000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    <b>AAV vectors quality control assays:</b> plasmid characterization, genome copy number titration, infectious titer determination (Taqman TCID50), purity (SDS-PAGE), endotoxin assay (Limulus Amebocyte Lysate) <b>Ad vectors quality control assays:</b> physical particle number determination, infectious titer determination (Taqman TCID50), replication-competant adenovirus (RCA) assay, genome structure analysis, endotoxin assay (Limulus Amebocyte Lysate) <b>Lentiviral/retroviral vectors quality control assays:</b> infectious DNA titer assay, replication-competent lentivirus (RCL), endotoxin assay (Limulus Amebocyte Lysate)
  2. Used by
    Vector Core (Penn)
  3. Website(s)
    http://www.med.upenn.edu/gtp/vectorcore/quality_control.shtml
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2012-12-04T11:37:29.286-05:00
  4. creator
    fcoldren
  5. modified
    2013-06-11T11:02:20.027-04:00

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