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Penn Gene Targeting Core and Laboratory (Penn)

eagle-i ID


Resource Type

  1. Core Laboratory


  1. Resource Description
    "The Penn Gene Targeting Core and Laboratory provides a truly complete knockout and knockin mouse service. In addition it provides all steps necessary for generation of knockout and knockin mutations in human embryonic stem cells (human ES cells) and human induced pluripotent stem cells (human IPS cells). For overview see Main Services below and for details see here. Depending on each project, PGT is using classical gene targeting methods or the revolutionary CRISPR/Cas9 – based system or a combination of both: • targeting vector design and construction • CRISPR/Cas9 design and construction • electroporation of the targeting vectors and/or CRISPR/Cas9 guide RNAs into mouse ES cells or human ES or IPS cells • PCR/Southern genotyping and karyotyping of the resulting ES/IPS clones • targeted mouse ES cell injection into blastocysts (usually together with the Transgenic and Chimeric Mouse Facility) It also provides general genomic Southern/PCR services for genotyping of mouse tails as well as any cell type from which sufficient genomic DNA can be obtained (human ES cells, human iPS cells, mesenchymal stem cells and others)."
  2. Additional Name
  3. Affiliation
    Department of Genetics, University of Pennsylvania
  4. Website(s)
  5. Director
    Raabe, Tobias D., Ph.D.
  6. Director
    Song, Wen-Chao, Ph.D.
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