Tissue microarray construction service

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "The pathology core can construct custom TMA's for researchers who have large collections of their tissue of interest and would like to be able to perform "high-throughput" analysis. <b><i>Why do I need a TMA?</i></b> • Basic research: Validate and verify gene expression data • Translational research: Diagnostic markers, prognostic markers, theraputic markers, response to therapy, query signaling pathways, drug discovery, tissue is a valuable resource <b><i>What do I need to make a TMA?</i></b> • Source tissue: animal tissue, clinical archival tissue, prospectively acquired clinical tissue, associated data • Pathologist: Confirm diagnosis, select representative areas for sampling (<i>TMA cores are representative: 20 studies to date on a variety of tumors have shown strong correspondence in IHC results on TMAs when compared to "large" sections (n=3-4 cores)</i>, validate the constructed array, score the array. • Antibody or in situ probe to query array: phosphorylation state specific antibodies, signaling pathways • Data management and analysis tools • Willingness to have <b><i>many collaborators</i></b> <b><i>TMA data analysis</i></b> • IHC scoring — 0 negative — 1 indeterminate — 2 weakly positive — 3 strongly positive • Scores for each individual core in TMA are collected for every stain performed • Large data sets • Expression data can be analyzed by bioinformatic methods developed for cDNA microarrays • Quantitative scoring (pixel counts/percent area stained) can also be performed as well" Arrays that have been constructed previously can be found on the core's website.

3. Service Fee URL

   http://pathcore.research.chop.edu/sites/default/files/files/2016CoreLabRateStructure%20.pdf

4. Additional Name

   TMA service

5. Related Resource

   0000013b-9b54-8f09-83a0-df0880000000

6. Service Provided by

   Pathology Core Laboratories (CHOP)

7. Website(s)

   http://pathcore.research.chop.edu/services/tissue-micro-array

8. Related Technique

   Clinical assay

9. Related Technique

   Tissue microarray assay