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Tissue microarray construction service
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000013b-9b56-6776-83a0-df0880000000
Resource Type
Properties
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Fee for service
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Yes
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Resource Description
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"The pathology core can construct custom TMA's for researchers who have large collections of their tissue of interest and would like to be able to perform "high-throughput" analysis.
<b><i>Why do I need a TMA?</i></b>
• Basic research: Validate and verify gene expression data
• Translational research: Diagnostic markers, prognostic markers, theraputic markers, response to therapy, query signaling pathways, drug discovery, tissue is a valuable resource
<b><i>What do I need to make a TMA?</i></b>
• Source tissue: animal tissue, clinical archival tissue, prospectively acquired clinical tissue, associated data
• Pathologist: Confirm diagnosis, select representative areas for sampling (<i>TMA cores are representative: 20 studies to date on a variety of tumors have shown strong correspondence in IHC results on TMAs when compared to "large" sections (n=3-4 cores)</i>, validate the constructed array, score the array.
• Antibody or in situ probe to query array: phosphorylation state specific antibodies, signaling pathways
• Data management and analysis tools
• Willingness to have <b><i>many collaborators</i></b>
<b><i>TMA data analysis</i></b>
• IHC scoring
— 0 negative
— 1 indeterminate
— 2 weakly positive
— 3 strongly positive
• Scores for each individual core in TMA are collected for every stain performed
• Large data sets
• Expression data can be analyzed by bioinformatic methods developed for cDNA microarrays
• Quantitative scoring (pixel counts/percent area stained) can also be performed as well"
Arrays that have been constructed previously can be found on the core's website.
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Service Fee URL
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http://pathcore.research.chop.edu/sites/default/files/files/2016CoreLabRateStructure%20.pdf
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Additional Name
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TMA service
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Related Resource
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0000013b-9b54-8f09-83a0-df0880000000
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Service Provided by
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Pathology Core Laboratories (CHOP)
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Website(s)
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http://pathcore.research.chop.edu/services/tissue-micro-array
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Related Technique
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Clinical assay
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Related Technique
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Tissue microarray assay
