Stem Cell & Xenograft Core (Penn)

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Resource Type

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1. Resource Description

   The Stem Cell and Xenograft Core (SCXC) is a comprehensive resource laboratory that integrates a viable tissue bank of normal human hematopoietic cells and hematopoietic malignancies with a full range of xenograft services. The SCXC is committed to facilitating and promoting translational research involving viable primary human hematopoietic tissues. Our core offers adult whole bone marrow from healthy donors and umbilical cord blood. Mononuclear and CD34+ cells from normal bone marrow and cord blood are available, and other cell fractions can be provided by arrangement. We maintain a large tissue bank of cells from hematopoietic malignancies including AML, ALL, CML, MDS and MPDs. All samples are fully annotated and frozen as viable cells. We also offer access to an immunomagnetic cell sorter (Miltneyi AutoMacs). Expertise in primary human hematopoietic stem/progenitor and leukemic cell culture and manipulation is available. For consultation or questions, please contact Martin Carroll. The SCXC offers a wide variety of xenograft services from training to full-service experiments. The Core maintains a large breeding colony of immune-deficient (NSG) mice for users xenograft studies. We also offer human immune system (CD34-transplanted) NSG mice for a wide variety of studies ranging from gene therapy to HIV. Experimental animals are housed in dedicated BSL-2 animal barrier space equipped for whole body irradiation and all necessary procedures and survival surgeries. Currently established xenograft models include normal human CD34 and leukemia engraftment, human iPS and ES-derived teratomas, human skin grafting, orthotopic human ovarian, hepatic and pancreatic tumor cell injections, renal capsule implantation. We also offer access to a dedicated optical/fluorescence (IVIS Spectrum) imaging system located within the Core's BSL-2 space. For consultation or questions, please contact Gwenn Danet-Desnoyers. Pennkey required to request services, contact to acquire a guest Pennkey if needed.

2. Contact

   Danet-Desnoyers, Gwenn., PhD

3. Affiliation

   Perelman School of Medicine

4. Affiliation

   Biomedical Research Core Facilities

5. Performs Technique

   Mouse breeding

6. Performs Technique

   Blood sampling

7. Performs Technique

   Biological sample processing

8. Performs Technique

   Rodent irradiation

9. Performs Technique

   Animal handling

10. Performs Technique

    Clinical assay

11. Performs Technique

    Imaging technique

12. Performs Technique

    Cell storage

13. Performs Technique

    Bone marrow aspiration

14. Performs Technique

    Isolation of cell population

15. Website(s)

    https://pathbio.med.upenn.edu/pbr/portal/scxc/

16. Website(s)

    https://www.med.upenn.edu/cores/stem_cell_and_xenograft.html

17. Director

    Danet-Desnoyers, Gwenn., PhD

18. Director

    Carroll, Martin., MD

19. Director

    Shan, Xiaochuan., MD, PhD

20. Secondary affiliation

    Abramson Cancer Center