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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    J1 embryonic stem cells were electroporated with NotI-digested Akt1 targeting vector and subjected to selection with G418, and the clones were screened for homologous recombination by PCR amplification of the intron 4 region and Southern blot analysis. Cells that were heterozygous for loxP sites flanking exons 3 and 4 of the Akt1 gene were injected into blastocysts derived from C57BL/6J mice and implanted into pseudopregnant CD-1 foster mothers according to standard methods. Chimeric mice (>90% as judged by coat color) were then bred with C57BL/6J mice. This mouse strain can be used to generate tissue-specific Akt1 deletions.
  2. Related Disease
    diabetes mellitus
  3. Related Disease
  4. Related Publication or Documentation
    Insulin regulates liver metabolism in vivo in the absence of hepatic Akt and Foxo1
  5. Related Publication or Documentation
    Loss of Akt1 in Mice Increases Energy Expenditure and Protects against Diet-Induced Obesity
  6. Parental Strain Name
  7. Genetic Alteration(s)
    Floxed Akt1
  8. Transgenic Construct
    Akt1 targeting vector
  9. Location
    Birnbaum Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016