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Akt1loxP/loxP

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013c-264c-e5ca-f162-a2b280000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    J1 embryonic stem cells were electroporated with NotI-digested Akt1 targeting vector and subjected to selection with G418, and the clones were screened for homologous recombination by PCR amplification of the intron 4 region and Southern blot analysis. Cells that were heterozygous for loxP sites flanking exons 3 and 4 of the Akt1 gene were injected into blastocysts derived from C57BL/6J mice and implanted into pseudopregnant CD-1 foster mothers according to standard methods. Chimeric mice (>90% as judged by coat color) were then bred with C57BL/6J mice. This mouse strain can be used to generate tissue-specific Akt1 deletions.
  2. Related Disease
    diabetes mellitus
  3. Related Disease
    obesity
  4. Related Publication or Documentation
    Insulin regulates liver metabolism in vivo in the absence of hepatic Akt and Foxo1
  5. Related Publication or Documentation
    Loss of Akt1 in Mice Increases Energy Expenditure and Protects against Diet-Induced Obesity
  6. Parental Strain Name
    C57BL6
  7. Genetic Alteration(s)
    Floxed Akt1
  8. Transgenic Construct
    Akt1 targeting vector
  9. Location
    Birnbaum Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-01-10T16:11:26.986-05:00
  4. creator
    fcoldren
  5. modified
    2013-09-09T09:03:02.266-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016