Crk(loxP/loxP);CrkL(loxP/loxP);Nestin-Cre

eagle-i ID

Resource Type

Properties

1. Resource Description

   The <i>CrkL</i> floxed allele carries three loxP sites, since the <i>Neo</i>TK gene is present, whereas only two loxP sites, flanking the first exon, are present in the <i>Crk</i> floxed allele. Crk is completely absent and CrkL expression is dramatically reduced in the cerebellum. Crk is not expressed in the hippocampus and CrkL expression decreased dramatically. Some cells, including pyramidal neurons in the CA3 region of the hippocampus, retained mild CrkL expression.

2. Additional Name

   <i>Crk^FL/FL;CrkL^FL/FL;Nestin-Cre</i>

3. Additional Name

   Neuron-specific <i>Crk</i> and <i>CrkL</i> knockout

4. Additional Name

   dcKO

5. Related Publication or Documentation

   Crk and Crk-like play essential overlapping roles downstream of disabled-1 in the Reelin pathway

6. Parental Strain Name

   C57BL/6 x 129SvEv

7. Biological process studied

   Brain development

8. Genetic Alteration(s)

   Floxed Crk

9. Genetic Alteration(s)

   Floxed CrkL

10. Genetic Alteration(s)

    Nestin-Cre

11. Phenotype Findings

    Small brain size

12. Phenotype Findings

    Postnatal growth retardation

13. Phenotype Findings

    Cerebellar hypofoliation

14. Phenotype Findings

    Abnormal Purkinje cell migration

15. Phenotype Findings

    Abnormal hippocampus pyramidal cell layer

16. Phenotype Findings

    Abnormal cerebral cortex morphology

17. Phenotype Findings

    Abnormal cortical lamination

18. Phenotype Findings

    Abnormal dentate gyrus morphology

19. Location

    Curran Laboratory