MTS knockin mice

eagle-i ID

Resource Type

Properties

1. Resource Description

   "Base pairs 1–105 of murine LAT cDNA were fused to LCP2 exon 1 and intron 1 DNA sequences. The LCP2 ATG was mutated, and the resulting fragment was cloned into the pPNT-FRT vector adjacent to the FRT-flanked neoR cassette. The targeting construct was electroporated into R1 embryonic stem cells, and injected into B6 × 129 blastocysts. The neoR cassette was excised by breeding to FLPe mice."

2. Related Publication or Documentation

   Mislocalization of SLP-76 leads to aberrant inflammatory cytokine and autoantibody production

3. Related Publication or Documentation

   Cutting edge: rescue of pre-TCR but not mature TCR signaling in mice expressing membrane-targeted SLP-76

4. Parental Strain Name

   B6.129

5. Biological process studied

   Immune system process

6. Genetic Alteration(s)

   Lcp2[tm5(Lat/Lcp2)Gak]

7. Phenotype Findings

   Impaired T cell development

8. Location

   Koretzky Laboratory