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Laser capture service

eagle-i ID


Resource Type

  1. Material processing service


  1. Fee for service
  2. Resource Description
    "The PALM system from Zeiss allows users to microdissect individual cells or regions of tissue from their slides for downstream molecular analysis. <b>Why use laser dissection?</b> Heterogeneous Nature of Tissue • Tumor Cells • Stroma • Lymphocytes Heterogeneous tissue may confound molecular analysis because it is impossible to determine which cells contribute to which cellular constituent of a given lysate. LMPC gives you the ability to isolate specific cells of interest without contamination from surrounding cells. <b>Specimen requirements</b> • OCT embedded frozen tissues • Paraffin embedded tissues • Cytology Preparations (i.e. blood smears, etc.) • Live Cell Cultures <b>Isolation of DNA</b> • Mutation analysis • Whole Genome Amplification • Chromosome Spreads • Select individual chromosomes for sequencing of translocation break points <b>Isolation of RNA from cryosections</b> • Quantitative RT-PCR • cDNA array hybridization • Chromosome Spreads <b>Isolation of protein from specific cell populations</b> • Amplification is not possible with protein samples so larger samples are needed • Highly sensitive methods of protein analysis (SELDI,MALD) are now available."
  3. Service Fee URL
  4. Related Resource
    Zeiss PALM Laser Capture Microdissection Microscope
  5. Service Provided by
    Pathology Core Laboratories (CHOP)
  6. Related Technique
    Laser capture microdissection
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
    ggrant (Gregory Grant)
  3. created
  4. creator
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016