Toluidine Blue Staining for Mast Cells Protocol

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Resource Type

Properties

1. Resource Description

   "Mast cells will stain metachromatically with toluidine blue; that is, they will stain a different color form the dye solution and the rest of the tissue. The control section should show mast cells stained red-purpose (metachromatic staining). The color shift, called metachromasia, generally is attributed to the cationic or basic dye and is somewhat dependent on pH, dye concentration, and temperature. Blue or violet dyes will show a red color shift, and red dyes will show a yellow color shift with metachromatic tissue elements."

2. Uses

   Staining

3. Used by

   Pathology Core Laboratories (CHOP)

4. Website(s)

   http://pathcore.research.chop.edu/docs/ToluidineBlueMastCells.pdf