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Toluidine Blue Staining for Mast Cells Protocol

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013d-a1c8-459b-2ed7-105480000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    "Mast cells will stain metachromatically with toluidine blue; that is, they will stain a different color form the dye solution and the rest of the tissue. The control section should show mast cells stained red-purpose (metachromatic staining). The color shift, called metachromasia, generally is attributed to the cationic or basic dye and is somewhat dependent on pH, dye concentration, and temperature. Blue or violet dyes will show a red color shift, and red dyes will show a yellow color shift with metachromatic tissue elements."
  2. Uses
    Staining
  3. Used by
    Pathology Core Laboratories (CHOP)
  4. Website(s)
    http://pathcore.research.chop.edu/docs/ToluidineBlueMastCells.pdf
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-03-25T09:42:33.401-04:00
  4. creator
    fcoldren
  5. modified
    2016-06-29T17:35:02.936-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016