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H19∆DMD∆G

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013d-f99e-a631-2ed7-105480000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    Using gene targeting, 2.9 kb of sequence including most of the DMD including repeats R2-R4 and HS2, and the G-rich repeat element was deleted. ES cells were electroporated with linearized targeting vector, and clones were screened by G418 selction for a targeting event. Targeted clones were transiently transfected with a Cre recombinase-expressing plasmid for removal of the PGK-<i>neo</i> cassette. Cells from targeted ES cell clones were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice. Morula were preserved.
  2. Related Publication or Documentation
    Analysis of sequence upstream of the endogenous H19 gene reveals elements both essential and dispensable for imprinting
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
    H19[tm3Msb]
  5. Phenotype Findings
    Loss of imprinted expression
  6. Location
    Bartolomei Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-04-11T11:11:33.435-04:00
  4. creator
    fcoldren
  5. modified
    2013-04-24T10:48:30.856-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016