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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    Using gene targeting, 1.3 kb of sequence including the G-rich repeat element was deleted. ES cells were electroporated with linearized targeting vector, and clones were screened by G418 selction for a targeting event. Targeted clones were transiently transfected with a Cre recombinase-expressing plasmid for removal of the PGK-<i>neo</i> cassette. Cells from targeted ES cell clones were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice.
  2. Related Publication or Documentation
    Analysis of sequence upstream of the endogenous H19 gene reveals elements both essential and dispensable for imprinting
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
  5. Phenotype Findings
    No change in imprinted expression
  6. Location
    Bartolomei Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016