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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    Using gene targeting, 3.8 kb of sequence containing the entire DMD and G-rich repeat element was deleted. ES cells were electroporated with linearized targeting vector, and clones were screened by G418 selction for a targeting event. Cells from targeted ES cell clones were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice. Mice harboring the H19<sup>Δ3.8kb-5′H19neoR</sup> allele were mated to transgenic mice expressing Cre recombinase for germ line excision of the PGK-<i>neo</i> cassette. This line is maintained on C57BL/6 background.
  2. Related Publication or Documentation
    Analysis of sequence upstream of the endogenous H19 gene reveals elements both essential and dispensable for imprinting
  3. Related Publication or Documentation
    Developmental profile of H19 differentially methylated domain (DMD) deletion alleles reveals multiple roles of the DMD in regulating allelic expression and DNA methylation at the imprinted H19/Igf2 locus
  4. Biological process studied
    Regulation of gene expression, epigenetic
  5. Genetic Alteration(s)
  6. Phenotype Findings
    Loss of imprinted expression
  7. Location
    Bartolomei Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016