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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    ES cells were electroporated with linearized targeting vector, and clones were screened by G418 selection for a targeting event that replaced a region of <i>H19</i> from approximately -2 to -4kb relative to the transcription start site (the DMD domain) with a <i>lox</i>P-flanked neomycin resistance cassette. Cre-mediated recombination removed the neomycin cassette. Cells from targeted ES cell clones were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice. Morula were preserved.
  2. Related Publication or Documentation
    Deletion of the H19 differentially methylated domain results in loss of imprinted expression of H19 and Igf2
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
  5. Phenotype Findings
    Loss of imprinted expression
  6. Phenotype Findings
    Decreased body weight
  7. Phenotype Findings
    Increased body size
  8. Location
    Bartolomei Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016