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H19ICR∆IVS

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013d-f9b0-ce08-2ed7-105480000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    The H19<sup><i>ICR∆IVS</i></sup> deletion removes ~0.9 kb of sequence between CTCF sites 2 and 3 at the ICR, which reduces the size of the ICR by ~50% and deletes ~35% of the CpGs at the ICR. Targeting vectors were linearized and electroporated into E14.1 ES cells. Correctly targeted ES cell clones were injected into C57BL/6J blastocysts and mice were generated by the Transgenic & Chimeric Mouse Facility at the University of Pennsylvania. Chimeras were obtained and mated to C57BL/6J mice. The <i>neo</i><sup>r</sup> cassette (flanked by <i>lox</i>P sites) was excised by crossing heterozygous mutant mice (H19<sup>ICRΔIVSneo/+</sup>) to mice expressing Cre recombinase under the control of the human cytomegalovirus promoter on a C57BL/6J genetic background (obtained from Edward Morissey).
  2. Related Publication or Documentation
    Novel cis-regulatory function in ICR-mediated imprinted repression of H19
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
    H19[ICR∆IVS]
  5. Phenotype Findings
    Loss of imprinted expression
  6. Location
    Bartolomei Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-04-11T11:24:05.284-04:00
  4. creator
    fcoldren
  5. modified
    2013-04-24T10:46:43.102-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016