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H19ICR-8nrCG

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013d-f9b2-d3b3-2ed7-105480000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    The <i>H19<sup>ICR-8nrCG</sup></i> contains mutations in 8 CpGs at the ICR, which depletes 16% of CpGs without changing the size of the ICR or disrupting CTCF sites. Targeting vectors were linearized and electroporated into E14.1 ES cells. Correctly targeted ES cell clones were injected into C57BL/6J blastocysts and mice were generated by the Transgenic & Chimeric Mouse Facility at the University of Pennsylvania. Chimeras were obtained and mated to C57BL/6J mice. The <i>neo</i><sup>r</sup> cassette (flanked by <i>lox</i>P sites) was excised in the mouse by crossing heterozygous mutant mice (<i>H19<sup>ICR-8nrCG/+</sup></i>) to mice expressing Cre recombinase under the control of the human cytomegalovirus promoter on a C57BL/6 genetic background (obtained from Edward Morissey).
  2. Related Publication or Documentation
    Novel cis-regulatory function in ICR-mediated imprinted repression of H19
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
    H19[ICR-8nrCG]
  5. Phenotype Findings
    Loss of imprinted expression
  6. Location
    Bartolomei Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-04-11T11:26:02.638-04:00
  4. creator
    fcoldren
  5. modified
    2013-04-24T10:46:26.170-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016