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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    Nine of the ten CpGs in the DMD were eliminated by introducing point mutations into the four 21-bp repeats (R1-R4) by gene targeting. Embryonic day 14 ES were electroporated with the linearized targeting vector and clones were screened by G418 selection for a targeting event with a <i>lox</i>P-flanked neomycin resistance cassette. The <i>neo</i><sup>r</sup> cassette was removed with Cre-mediated recombination by transiently transfecting the targeted ES cells with pTurboCre plasmid. Cells from targeted ES cell clones were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice.
  2. Related Publication or Documentation
    Antagonism between DNA hypermethylation and enhancer-blocking activity at the H19 DMD is uncovered by CpG mutations
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
  5. Phenotype Findings
    Paternal H19 expression
  6. Location
    Bartolomei Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016