eagle-i The University of PennsylvaniaThe University of Pennsylvania
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

H19DMD-9CG

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013d-f9d0-c50c-2ed7-105480000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    Nine of the ten CpGs in the DMD were eliminated by introducing point mutations into the four 21-bp repeats (R1-R4) by gene targeting. Embryonic day 14 ES were electroporated with the linearized targeting vector and clones were screened by G418 selection for a targeting event with a <i>lox</i>P-flanked neomycin resistance cassette. The <i>neo</i><sup>r</sup> cassette was removed with Cre-mediated recombination by transiently transfecting the targeted ES cells with pTurboCre plasmid. Cells from targeted ES cell clones were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice.
  2. Related Publication or Documentation
    Antagonism between DNA hypermethylation and enhancer-blocking activity at the H19 DMD is uncovered by CpG mutations
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
    H19[DMD-9CG]
  5. Phenotype Findings
    Paternal H19 expression
  6. Location
    Bartolomei Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-04-11T11:58:28.122-04:00
  4. creator
    fcoldren
  5. modified
    2013-04-24T10:45:51.374-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016