eagle-i The University of PennsylvaniaThe University of Pennsylvania
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.


eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    Using gene targeting, a 2.5-kb region that contains the <i>H19</i> endodermal enhancers was replaced with a fragment that harbors the <i>Afp</i> enhancers. The targeting vector was electroporated into E14 129 embryonic stem (ES) cells and neomycin-resistant clones were screened for a recombination event that introduced the <i>Afp</i> enhancer fragment and a cassette containing the neomycin resistance and thymidine kinase genes flanked by loxP sites (neoRtk) in place of the <i>H19</i> endodermal enhancers (H19<sup>Afpneo</sup>). These cells were subsequently transfected with Cre recombinase to delete the <i>neoRtk</i> genes (H19<sup>Afp</sup>). Cells from targeted ES cell clones (with and without the neo r gene) were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice. Morula and sperm were preserved.
  2. Related Publication or Documentation
    Role of H19 3' sequences in controlling H19 and Igf2 imprinting and expression
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
  5. Phenotype Findings
    H19 paternal expression in liver
  6. Location
    Bartolomei Laboratory
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
  3. created
  4. creator
  5. modified
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016