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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    Using gene targeting, the 4.2-kb domain between the end of the H19 transcription unit and the endodermal enhancers from a normally imprinted H19 transgene was removed in this mouse line. The targeting vector was electroporated and clones were screened by G418 selction for a targeting event that introduced a loxP site 5′ of the 4.2-kb region and the neoRtk cassette 3′ (H19Δ4.2neo). H19Δ4.2neo ES cells were subsequently electroporated with Cre and screened by Southern hybridization for deletion of the 4.2-kb domain and the neoRtk cassette, leaving one loxP site in this region (H19Δ4.2). Cells from targeted ES cell clones were injected into C57BL/6J blastocysts, and the blastocysts were transferred to pseudopregnant female mice.
  2. Related Publication or Documentation
    Role of H19 3' sequences in controlling H19 and Igf2 imprinting and expression
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
  5. Phenotype Findings
    No change in imprinted expression
  6. Location
    Bartolomei Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016