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MBD3

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013d-fa21-828c-2ed7-105480000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    This line was generated by the group of Adrian Bird. Targeted deletion of <i>Mbd3</i> was acheived by replacing a genomic fragment containing exons 2-7 of the <i>Mbd3</i> gene with a promoterless beta-geo cassette in embryonic stem cells. This removed sequences encoding all but the N-terminal 36 amino acids of the protein. Gene targeting was performed in the ES cell line E14 TG2a, which is derived from the mouse substrain 129/Ola. ES cells were transfected with the linearized targeting vector by electroporation. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric pups were mated with C57BL/6 mice. The line is maintained by intercrossing heterozygotes.
  2. Related Publication or Documentation
    Closely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse development
  3. Parental Strain Name
    129/Ola
  4. Parental Strain Name
    C57BL/6
  5. Genetic Alteration(s)
    Mbd3[-]
  6. Phenotype Findings
    Complete prenatal lethality
  7. Location
    Bartolomei Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-04-11T13:26:33.945-04:00
  4. creator
    fcoldren
  5. modified
    2013-04-24T10:23:17.522-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016