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Zp3dsMBD3

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013d-fa23-4490-2ed7-105480000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    DNA was injected into pronuclei of fertilized one-cell mouse eggs derived from (C57BL/6 x SJL)F<sub>1</sub> intercrosses. Mice were maintained on a C57BL/6J background. In this mouse line, RNAi utilizes a transgene to reduce Mbd3 RNA levels. The transgene employs the zona pellucida 3 (<i>Zp3</i>) promoter which drives expression of linked sequences in growing oocytes. The promoter is upstream of an <i>Mbd3</i> inverted repeat that generates an approximately 510-bp dsRNA.
  2. Related Publication or Documentation
    Maintenance of paternal methylation and repression of the imprinted H19 gene requires MBD3
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
    Zp3-dsMBD3
  5. Phenotype Findings
    Loss of imprinted expression
  6. Location
    Bartolomei Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-04-11T13:30:38.111-04:00
  4. creator
    fcoldren
  5. modified
    2013-04-24T10:51:06.427-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016