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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    DNA was injected into pronuclei of fertilized one-cell mouse eggs derived from (C57BL/6 x SJL)F<sub>1</sub> intercrosses. Mice were maintained on a C57BL/6J background. In this mouse line, RNAi utilizes a transgene to reduce Mbd3 RNA levels. The transgene employs the zona pellucida 3 (<i>Zp3</i>) promoter which drives expression of linked sequences in growing oocytes. The promoter is upstream of an <i>Mbd3</i> inverted repeat that generates an approximately 510-bp dsRNA.
  2. Related Publication or Documentation
    Maintenance of paternal methylation and repression of the imprinted H19 gene requires MBD3
  3. Biological process studied
    Regulation of gene expression, epigenetic
  4. Genetic Alteration(s)
  5. Phenotype Findings
    Loss of imprinted expression
  6. Location
    Bartolomei Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016