Crry(-/-)/C3(-/-)

eagle-i ID

Resource Type

Properties

1. Resource Description

   These mice were a gift from Dr. Hector Molina. Insertion of a neomycin resistance cassette in exon 5 resulted in mice deficient in <i>Crry</i>. Heterozygous mice, obtained from gene targeting in embryonic stem cells and blastocyst injection, were crossed with C3^-/- mice and subsequently intercrossed to generate Crry^-/-C3^-/- mice. Crry and C3 are absent from platelets.

2. Additional Name

   Crl1(-/-);C3(-/-)

3. Related Publication or Documentation

   Crry, but not CD59 and DAF, is indispensable for murine erythrocyte protection in vivo from spontaneous complement attack

4. Related Publication or Documentation

   Activator-specific requirement of properdin in the initiation and amplification of the alternative pathway complement

5. Related Publication or Documentation

   Deficiency of decay-accelerating factor and complement receptor 1–related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg

6. Related Publication or Documentation

   Complement-mediated clearance of erythrocytes: mechanism and delineation of the regulatory roles of Crry and DAF. Decay-accelerating factor

7. Related Publication or Documentation

   Deletion of Crry and DAF on murine platelets stimulates thrombopoiesis and increases factor H-dependent resistance of peripheral platelets to complement attack

8. Related Publication or Documentation

   Retrovirus-mediated over-expression of decay-accelerating factor rescues Crry-deficient erythrocytes from acute alternative pathway complement attack

9. Related Publication or Documentation

   A critical role for murine complement regulator crry in fetomaternal tolerance

10. Website(s)

    http://www.informatics.jax.org/allele/genoview/MGI:3505876?counter=8

11. Parental Strain Name

    mixed 129/C57BL/6

12. Biological process studied

    Immune system process

13. Genetic Alteration(s)

    Cr1l[tm1Hmo]

14. Genetic Alteration(s)

    C3[tm1Hrc]

15. Phenotype Findings

    Normal survival

16. Phenotype Findings

    Abnormal complement pathway

17. Location

    Wen-Chao Song Laboratory