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C5aR GFP-KI

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013e-5c26-bcec-2f2e-ad3380000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    "A loxP site was inserted upstream of exon 2. A cassette containing (5' to 3') an internal ribosomal entry site (IRES), EGFP, SV40 polyadenlyation sequence, FRT-flanked neo cassette and loxP site was inserted into the 3' UTR." This strain is cryo-preserved. Gene targeting in embryonic stem (ES) cells was performed according to standard protocols. Linearized targeting vector was transfected into ES cells (TL-1, a cell line derived from the 129/SvEv mouse strain). The neomycin and diphtheria toxin genes on the vector allowed for double selection of ES cells that underwent homologous recombination and incorporated GFP and loxP into the <i>C5ar1</i> gene locus on one strand of their genomic DNA. Positive clones were expanded, confirmed in second-round screening, and microinjected into C57BL/6 mouse blastocysts before being transferred to pseudopregnant females. Male chimeras were mated subsequently with female C57BL/6 mice (The Jackson Laboratory) to achieve germline transmission. "GFP is expressed highly on Gr-1<sup>+</sup>CD11b<sup>+</sup> cells in the blood, spleen and bone marrow, and moderately on CD11b<sup>+</sup>F4/80<sup>+</sup> circulating leukocytes and elicited peritoneal macrophages. No GFP is detected on resting or activated T lymphocytes, nor on splenic myeloid or plasmacytoid dendritic cells."
  2. Additional Name
    C5aR GFP knock-in
  3. Related Publication or Documentation
    C5aR expression in a novel GFP reporter gene knockin mouse: implications for the mechanism of action of C5aR signaling in T cell immunity
  4. Website(s)
    http://www.informatics.jax.org/javawi2/servlet/WIFetch?page=alleleDetail&key=819124
  5. Website(s)
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324670/#!po=37.5000
  6. Related Technique
    Transgenic mouse model generation
  7. Parental Strain Name
    C57BL/6
  8. Biological process studied
    gene expression
  9. Genetic Alteration(s)
    C5ar1[tm1Song]
  10. Phenotype Findings
    Abnormal neutrophil physiology
  11. Phenotype Findings
    Abnormal protein targeting
  12. Location
    Wen-Chao Song Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-04-30T14:15:31.470-04:00
  4. creator
    fcoldren
  5. modified
    2016-02-17T17:51:13.403-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016