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eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    "A loxP site was inserted upstream of exon 2. A cassette containing (5' to 3') an internal ribosomal entry site (IRES), EGFP, SV40 polyadenlyation sequence, FRT-flanked neo cassette and loxP site was inserted into the 3' UTR." This strain is cryo-preserved. Gene targeting in embryonic stem (ES) cells was performed according to standard protocols. Linearized targeting vector was transfected into ES cells (TL-1, a cell line derived from the 129/SvEv mouse strain). The neomycin and diphtheria toxin genes on the vector allowed for double selection of ES cells that underwent homologous recombination and incorporated GFP and loxP into the <i>C5ar1</i> gene locus on one strand of their genomic DNA. Positive clones were expanded, confirmed in second-round screening, and microinjected into C57BL/6 mouse blastocysts before being transferred to pseudopregnant females. Male chimeras were mated subsequently with female C57BL/6 mice (The Jackson Laboratory) to achieve germline transmission. "GFP is expressed highly on Gr-1<sup>+</sup>CD11b<sup>+</sup> cells in the blood, spleen and bone marrow, and moderately on CD11b<sup>+</sup>F4/80<sup>+</sup> circulating leukocytes and elicited peritoneal macrophages. No GFP is detected on resting or activated T lymphocytes, nor on splenic myeloid or plasmacytoid dendritic cells."
  2. Additional Name
    C5aR GFP knock-in
  3. Related Publication or Documentation
    C5aR expression in a novel GFP reporter gene knockin mouse: implications for the mechanism of action of C5aR signaling in T cell immunity
  4. Website(s)
  5. Website(s)
  6. Related Technique
    Transgenic mouse model generation
  7. Parental Strain Name
  8. Biological process studied
    gene expression
  9. Genetic Alteration(s)
  10. Phenotype Findings
    Abnormal neutrophil physiology
  11. Phenotype Findings
    Abnormal protein targeting
  12. Location
    Wen-Chao Song Laboratory
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016