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C5aR GFP-KI
eagle-i ID
http://eagle-i.itmat.upenn.edu/i/0000013e-5c26-bcec-2f2e-ad3380000000
Resource Type
Properties
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Resource Description
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"A loxP site was inserted upstream of exon 2. A cassette containing (5' to 3') an internal ribosomal entry site (IRES), EGFP, SV40 polyadenlyation sequence, FRT-flanked neo cassette and loxP site was inserted into the 3' UTR."
This strain is cryo-preserved.
Gene targeting in embryonic stem (ES) cells was performed according to standard protocols. Linearized targeting vector was transfected into ES cells (TL-1, a cell line derived from the 129/SvEv mouse strain). The neomycin and diphtheria toxin genes on the vector allowed for double selection of ES cells that underwent homologous recombination and incorporated GFP and loxP into the <i>C5ar1</i> gene locus on one strand of their genomic DNA. Positive clones were expanded, confirmed in second-round screening, and microinjected into C57BL/6 mouse blastocysts before being transferred to pseudopregnant females. Male chimeras were mated subsequently with female C57BL/6 mice (The Jackson Laboratory) to achieve germline transmission.
"GFP is expressed highly on Gr-1<sup>+</sup>CD11b<sup>+</sup> cells in the blood, spleen and bone marrow, and moderately on CD11b<sup>+</sup>F4/80<sup>+</sup> circulating leukocytes and elicited peritoneal macrophages. No GFP is detected on resting or activated T lymphocytes, nor on splenic myeloid or plasmacytoid dendritic cells."
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Additional Name
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C5aR GFP knock-in
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Related Publication or Documentation
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C5aR expression in a novel GFP reporter gene knockin mouse: implications for the mechanism of action of C5aR signaling in T cell immunity
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Website(s)
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http://www.informatics.jax.org/javawi2/servlet/WIFetch?page=alleleDetail&key=819124
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Website(s)
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324670/#!po=37.5000
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Related Technique
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Transgenic mouse model generation
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Parental Strain Name
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C57BL/6
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Biological process studied
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gene expression
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Genetic Alteration(s)
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C5ar1[tm1Song]
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Phenotype Findings
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Abnormal neutrophil physiology
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Phenotype Findings
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Abnormal protein targeting
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Location
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Wen-Chao Song Laboratory
