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MudPIT (LC/LC-MS/MS) analyis–comprehensive analysis of a subproteome or proteome

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-6812-c631-a468-831a80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "This service is analogous to Gel/LC-MS/MS except that the subproteome or proteome is digested with a protease in solution rather than separation on a gel, and the digest then is fractionated by ion exchange chromatography prior to LC-MS/MS analysis as described above. This method typically yields less protein identifications and sequence coverage compared with Gel/LC-MS/MS when the same number of fractions and LC-MS/MS runs are used. In addition, there is no information concerning approximate Molecular Weight of the identified proteins. Hence, Gel/LC-MS/MS is recommended for most subproteome and proteome analyses. Nonetheless, MudPIT can be used as needed in special situations."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/user-fees
  4. Related Resource
    Thermo Fisher Hybrid LTQ-OrbitrapXL™ mass spectrometer with online Eksigent® Nano-capillary HPLC
  5. Related Resource
    Applied Biosystems® 5500 Q TRAP® Mass Spectrometer with online Waters NanoACQuity® Nano-capillary HPLC
  6. Service Provided by
    Proteomics Facility (Wistar)
  7. Website(s)
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/services
  8. Related Technique
    Protein identification
  9. Related Technique
    Liquid chromatography-tandem mass spectrometry
  10. Related Technique
    Ion chromatography
  11. Related Technique
    Mass spectrometry data analysis
  12. Related Technique
    Trypsination
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-21T14:52:28.688-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:59:10.029-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016