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MudPIT (LC/LC-MS/MS) analyis–comprehensive analysis of a subproteome or proteome

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-6812-c631-a468-831a80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "This service is analogous to Gel/LC-MS/MS except that the subproteome or proteome is digested with a protease in solution rather than separation on a gel, and the digest then is fractionated by ion exchange chromatography prior to LC-MS/MS analysis as described above. This method typically yields less protein identifications and sequence coverage compared with Gel/LC-MS/MS when the same number of fractions and LC-MS/MS runs are used. In addition, there is no information concerning approximate Molecular Weight of the identified proteins. Hence, Gel/LC-MS/MS is recommended for most subproteome and proteome analyses. Nonetheless, MudPIT can be used as needed in special situations."
  3. Service Fee URL
    https://wistar.org/research-discoveries/shared-resources/proteomics-metabolomics-facility
  4. Related Resource
    Thermo Fisher Hybrid LTQ-OrbitrapXL™ mass spectrometer with online Eksigent® Nano-capillary HPLC
  5. Related Resource
    Applied Biosystems® 5500 Q TRAP® Mass Spectrometer with online Waters NanoACQuity® Nano-capillary HPLC
  6. Service Provided by
    Proteomics and Metabolomics Facility (Wistar)
  7. Website(s)
    https://wistar.org/research-discoveries/shared-resources/proteomics-metabolomics-facility
  8. Related Technique
    Protein identification
  9. Related Technique
    Liquid chromatography-tandem mass spectrometry
  10. Related Technique
    Ion chromatography
  11. Related Technique
    Mass spectrometry data analysis
  12. Related Technique
    Trypsination
 
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Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016