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Reverse phase microbore HPLC peptide mapping service

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    "Purpose: High-resolution, reversed-phase, chromatographic separation of in-gel tryptic digest for comparative mapping of two or more gel bands. Comparative mapping is the most quantitative method for detecting differences and similarities between different bands on a gel such as chemical or post-translational modifications, proteolytic processing, etc. Requirements: Tryptic digest performed in this facility of gel bands containing at least 10 pmoles of target protein(s). Results: Reproducible peptide maps monitored at 215 nm with peaks collected into pre-cleaned microcentrifuge tubes for subsequent analysis of selected peaks (minimum of 10) by MALDI MS."
  3. Service Fee URL
  4. Related Resource
    Agilent 1100 Microbore HPLC System
  5. Service Provided by
    Proteomics and Metabolomics Facility (Wistar)
  6. Website(s)
  7. Related Technique
    High performance liquid chromatography
  8. Related Technique
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016