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Reverse phase microbore HPLC peptide mapping service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-6814-f32f-a468-831a80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Purpose: High-resolution, reversed-phase, chromatographic separation of in-gel tryptic digest for comparative mapping of two or more gel bands. Comparative mapping is the most quantitative method for detecting differences and similarities between different bands on a gel such as chemical or post-translational modifications, proteolytic processing, etc. Requirements: Tryptic digest performed in this facility of gel bands containing at least 10 pmoles of target protein(s). Results: Reproducible peptide maps monitored at 215 nm with peaks collected into pre-cleaned microcentrifuge tubes for subsequent analysis of selected peaks (minimum of 10) by MALDI MS."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/user-fees
  4. Related Resource
    Agilent 1100 Microbore HPLC System
  5. Service Provided by
    Proteomics Facility (Wistar)
  6. Website(s)
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/services#GEL
  7. Related Technique
    High performance liquid chromatography
  8. Related Technique
    Trypsination
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-21T14:55:33.634-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:59:12.296-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016