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MALDI mass spectrometry

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-6817-df3f-a468-831a80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Purpose: Determine masses of purified peptides or proteins in solution. MALDI mass analysis is feasible over a wide mass range and can help identify the extent of post-translational modifications such as oxidation, glycosylation, and phosphorylation. It is the method of choice to check integrity of synthetic peptides, peptides from fractions of HPLC separations, and integrity of recombinant proteins. While some salts and buffers are tolerated, it may be necessary to 'clean up' the sample prior to analysis if buffer components suppress the mass signal. Requirements: Purified peptides for routine analysis should be between 0.5-5 pmoles/µl in 0.1% TFA (sample also may contain up to 50% acetonitrile). Proteins should be 10 pmoles/µl, preferably in 20 mM NH4HCO3. It is especially important to avoid the following: Phosphate buffer >20 mM, most detergents >0.1% or SDS >0.01%, alkali metal salts >1M, glycerol >1%, Tris buffer >10 mM, guanidine or urea >1 M, or sodium azide >1 mM. Results: Printouts of the masses found and a printout of the standard used for the calibration of the instrument in PDF file format."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/user-fees
  4. Related Resource
    Applied Biosystems Voyager-DE® PRO MALDI TOF Mass Spectrometer
  5. Service Provided by
    Proteomics Facility (Wistar)
  6. Website(s)
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/services
  7. Related Technique
    Post translational modification identification by mass spectrometry
  8. Related Technique
    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-21T14:57:40.240-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:59:20.213-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016