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Post-translational modification identifications

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-681b-a09b-a468-831a80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Purpose: Identification and location of post-translational modifications such as phosphorylation, acetylation, etc. This can be accomplished using several services such as in-gel digestion, MALDI MS, LC-MS/MS, and RP-HPLC mapping, depending upon the type of project. Requirements: Project dependent (inquire with Facility Manager). Typically, an intact 1D or 2D gel stained with Coomassie® Blue R-250 or colloidal Coomassie® that was prepared following our "Sequence-quality SDS-gel guidelines," same as for LC-MS/MS. Results: Printouts of description of post-translational modifications found and supporting data in PDF file format."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/user-fees
  4. Related Resource
    Thermo Fisher Hybrid LTQ-OrbitrapXL™ mass spectrometer with online Eksigent® Nano-capillary HPLC
  5. Related Resource
    Applied Biosystems® 5500 Q TRAP® Mass Spectrometer with online Waters NanoACQuity® Nano-capillary HPLC
  6. Related Resource
    Applied Biosystems Voyager-DE® PRO MALDI TOF Mass Spectrometer
  7. Related Resource
    Agilent 1100 Microbore HPLC System
  8. Service Provided by
    Proteomics Facility (Wistar)
  9. Website(s)
    http://www.wistar.org/our-science/shared-facilities/proteomics-facility/services
  10. Related Technique
    High performance liquid chromatography
  11. Related Technique
    Liquid chromatography-tandem mass spectrometry
  12. Related Technique
    Matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  13. Related Technique
    Trypsination
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-21T15:03:33.180-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:59:20.980-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016