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Live-cell time-lapse microscopy

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-77f5-1317-a468-831a80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Using the Nikon TE300 inverted microscope in room 287 with its ImagePro workstation, 5D time-lapse studies of cells grown in culture can be accomplished (X, Y, time, wavelength and location). Imaging combinations using phase contrast and fluorescence illumination are possible, taken at short or long intervals, from several minutes to several days. The environmental chamber has temperature and CO2 control and is monitored continuously. Cells are normally presented in multi-well plates or 35mm glass-bottom dishes, but other vessels can be accommodated as needed. Common uses for this system include scratch (wound) assays and cellular proliferation studies. For higher resolution options, including 3D timelapse, The Leica SP5 II confocal microscope (see below) is also capable of multi-day timelapse, with CO2 and temperature control."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/microscopy-facility/user-fees
  4. Related Resource
    Nikon TE300 inverted microscope
  5. Service Provided by
    Imaging Facility (Wistar)
  6. Website(s)
    http://www.wistar.org/our-science/shared-facilities/microscopy-facility/equipment-and-services
  7. Related Technique
    Fast live imaging
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-24T16:54:19.883-04:00
  4. creator
    fcoldren
  5. modified
    2014-09-18T16:37:40.184-04:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016