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Two-photon microscopy

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    "2-photon microscopy continues where traditional confocal leaves off. With laser excitations in the longer wavelengths, 2P allows deeper imaging and reduced phototoxicity. This system was designed to be used for intravital imaging, with tissues such as inguinal lymph nodes, but it can also used with cell cultures in acceptable vessels, tissue slides, explanted specimens such as lymph nodes and brain slices, kept viable in heated media. This system has the ability to resolve non-labeled structural details with 2nd harmonics (SHG), as well as capture emission wavelengths simultaneously with three other detectors. Thick, fixed specimens, such as spheroids and skin reconstructs can benefit from the increased penetration and imaging capabilities of this system, as well as fixed cultures in deep collagen matrices."
  3. Service Fee URL
  4. Additional Name
    2-photon microscopy
  5. Related Resource
    Prairie Instruments Ultima two-photon microscope
  6. Service Provided by
    Imaging Facility (Wistar)
  7. Website(s)
  8. Related Technique
    Intravital microscopy
  9. Related Technique
    Multiphoton microscopy
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016