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Molecular Screening and Protein Expression Facility (Wistar)

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-8b15-e20f-a468-831a80000000

Resource Type

  1. Core Laboratory

Properties

  1. Resource Description
    The Molecular Screening and Protein Expression Shared Resource fosters collaboration by providing expertise in biochemical and cell-based assay development for high-throughput screening and compound profiling. Such assays enable researchers to identify small molecule compounds which interact with a target protein of interest. These compounds can then be used as tools to further study the target proteins function and signaling pathways in cells. The facility maintains a small molecule library of ~100,000 drug-like small molecules for high-throughput screening and provides access to liquid handling robotics and a multilabel plate reader. In addition, the facility also provides access to Biacore T200 SPR instrument for label-free binding kinetics and a high-content screening system. Guidance is provided for target justification, assay development, miniaturization, robotic automation, and adaptation to HTS-compatible, high-density microplate (384-well) formats. The facility provides production of recombinant proteins in insect cell systems and affinity purification of recombinant proteins upon request. The facility also maintains glycerol stocks of shRNA libraries, which can be accessed by investigators for use in target validation studies. Through our partnership with The Gene Editing Institute at Christiana Care, Wistar researchers have access to gene editing (CRISPR/Cas9) services. The Wistar Molecular Screening Facility was developed with support from the Commonwealth of Pennsylvania Department of Community and Economic Development Keystone Innovation Zone initiative, The F. M. Kirby Foundation, The CLAWS Foundation, The Florence & Daniel Green Foundation, The McClean Contributionship, From The Heart Foundation, the Noreen O’Neill Foundation for Melanoma Research, NIH shared instrumentation grants, and an NCI Cancer Center Support Grant.
  2. Affiliation
    The Wistar Institute
  3. Website(s)
    https://wistar.org/research-discoveries/shared-resources/molecular-screening-protein-expression-facility
  4. Director
    Salvino, Paul, Ph.D.
  5. Director
    Cassel, Joel
 
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Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016