eagle-i The University of PennsylvaniaThe University of Pennsylvania
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

High-throughput-screening service

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-8b40-1fc6-a468-831a80000000

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "<b>Pilot screening</b> After establishing an optimized assay protocol that meets acceptable criteria in a replicate plate test, a pilot screen of 2,000 to 3,000 compounds from a reference library with a high-degree of pharmacophore content is recommended. The purpose of the pilot screen is to assess the behavior of the assay conditions and automation procedures you have optimized during assay development under actual screening conditions. In addition, this pilot screen will be used to establish criteria for ‘hit’ conditions and selection. At this point, an assay protocol must be submitted for inclusion in our screening database. <b>Production screens</b> The facility has the capability to process upto ~100 microtiter plates per day depending on the assay type and number of steps. Facility staff will work with investigators/users to determine screen logistics (time considerations, libraries, amounts/volumes of reagents, etc). For newly developed assays, it is recommended that the assay conditions be tested in a pilot-scale screen (see above). For previously developed assays, demonstration of acceptable criteria in a single day replicate plate procedure will be sufficient to proceed with a production screen. When performing a production screen, it is preferable to process as many plates as possible during usage of the equipment and laboratory staff time. We strongly suggest screening all compounds in duplicate to enhance interpretation of data points. To schedule any screen, contact David C. Schultz. Do not make arrangements with staff of the facility. Please plan to schedule your screen well in advance of the date you would like to start your experiment to eliminate scheduling conflicts and to ensure that the libraries are properly formatted. Only screening facility personnel are permitted to handle library stock plates, and thus they perform all compound transfers from library plates into assay plates. Screening facility personnel will not be responsible for conducting any other parts of a screen unless agreed upon prior to the initiation of the screen, and will be charged as assisted use. The laboratory conducting the screen is responsible for purchasing all plasticware to be used for the screen, this includes pipette tips, plates, and any other materials specific to your screen (reagent troughs, plate seals, tissue culture reagents, assay reagents, labels for barcoding etc.). The screening laboratory has also negotiated some bulk discounting on plates and pipette tips for the liquid handling unit, and therefore, investigators can purchase these items from the screening facility at cost."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/molecular-screening-facility/cost-structure
  4. Related Resource
    NIH Clinical Collection
  5. Related Resource
    Spectrum Collection
  6. Related Resource
    Maybridge "HitFinder" Library
  7. Related Resource
    ChemDiv Discovery Chemistry Set
  8. Related Resource
    ChemDiv Target Specific Chemistry Set
  9. Related Resource
    Lankenau Chemical Genomics Center library
  10. Service Provided by
    Molecular Screening Facility (Wistar)
  11. Website(s)
    http://www.wistar.org/our-science/shared-facilities/molecular-screening-facility/services
  12. Related Technique
    Compound library screening
  13. Related Technique
    RNAi screening
  14. Related Technique
    Small molecule screening
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-28T10:49:50.778-04:00
  4. creator
    fcoldren
  5. modified
    2015-01-13T10:05:10.658-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016