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Recombinant plasmid DNA engineering

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-8bf2-e310-a468-831a80000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "The facility staff will assist users in designing a cloning strategy to engineer an appropriate recombinant plasmid DNA that meets the specific goals of the project. The facility supports conventional, ligation independent, Gateway, and site-directed mutagenesis cloning technologies. The nucleic acids (e.g., cDNA, promoter, shRNA, etc.) are isolated from a customer-supplied plasmid DNA(s) and subcloned into a new vector of choice. Newly derived plasmid DNAs are authenticated by restriction endonuclease digestion, DNA sequencing, and prepared for subsequent transfection based applications."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/protein-expression-and-libraries-facility/policies-and-user-fees
  4. Service Provided by
    Protein Expression Facility (Wistar)
  5. Website(s)
    http://www.wistar.org/our-science/shared-resources-cores/protein-expression-facility/services/recombinant-plasmid-dna-engi
  6. Related Technique
    Plasmid construction
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-28T14:05:30.866-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:54:07.827-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016