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Recombinant protein production via Baculovirus expression systems (BVES)

eagle-i ID

http://eagle-i.itmat.upenn.edu/i/0000013f-8bfa-0121-a468-831a80000000

Resource Type

  1. Material production service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Baculovirus expression systems (BVES) represent an alternative approach to produce large amounts of properly folded and functional recombinant proteins. The benefits of protein expression with baculovirus include the virus being able to accommodate large inserts, eukaryotic post-translational modification, enhanced protein folding and function, high expression levels, easy scale up with high-density suspension culture, and safety. The Facility has >20 years of experience in preparing baculovirus vectors from nearly all commercially available BVES. Investigators have the option to provide the facility with previously constructed baculovirus transfer vectors, have facility staff engineer the plasmid DNA construct, or to provide the facility with small volumes of previously prepared virus stock." Available services include: preparation of high-titer baculovirus stock, amplification of high-titer baculovirus stocks, analytical scale productions for optimization of protein expression, and preparative scale productions."
  3. Service Fee URL
    http://www.wistar.org/our-science/shared-facilities/protein-expression-and-libraries-facility/policies-and-user-fees
  4. Service Provided by
    Protein Expression Facility (Wistar)
  5. Website(s)
    http://www.wistar.org/our-science/shared-resources-cores/protein-expression-facility/services/recombinant-protein-producti
  6. Related Technique
    Recombinant protein production
 
RDFRDF
 
Provenance Metadata About This Resource Record
  1. workflow state
    Published
  2. contributor
    fcoldren
  3. created
    2013-06-28T14:11:38.218-04:00
  4. creator
    fcoldren
  5. modified
    2013-07-02T10:54:08.608-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016